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Effects of Indian almond leaf aqueous extract on Staphylococcus aureus in relation to concentration and exposure time |
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| รหัสดีโอไอ | |
| Creator | Jirapa Rungreangsakhon |
| Title | Effects of Indian almond leaf aqueous extract on Staphylococcus aureus in relation to concentration and exposure time |
| Contributor | Gun Kanchanacharoen, Natnaree Kaewsiri, Sitthichon Rattanachan |
| Publisher | Mahasarakham University |
| Publication Year | 2569 |
| Journal Title | Journal of Science and Technology Mahasarakham University |
| Journal Vol. | 45 |
| Journal No. | 3 |
| Page no. | 329-336 |
| Keyword | Indian almond leaf, Staphylococcus aureus, aqueous extract, tannin, antibacterial activity |
| URL Website | https://li01.tci-thaijo.org/index.php/scimsujournal |
| Website title | Journal of Science and Technology Mahasarakham University |
| ISSN | 1686-9664 (Print), 2586-9795(Online) |
| Abstract | Staphylococcus aureus is a pathogenic bacterium that causes skin infections in both pets and humans with the increasing trend of bacterial resistance to antibiotics, prompting interest in alternative antibacterial agents from natural sources. Therefore, this study aims to the efficacy of aqueous extract from Indian almond (Terminalia catappa Linn.) leaves, focusing on determining the minimum bactericidal concentration (MBC) and the time required for effective killing S. aureus. The experiment was conducted by preparing an aqueous extract of Indian almond leaves through boiling, followed by extended immersion for 7 days. The MBC and Time-dependent killing was performed by macrodilution method. The results show that the ferment extract of Indian almond leaf extract contained 0.76 mg/ml of tannin. The MBC of ferment extract against S. aureus was between 0.35 to 0.4 mg/ml. In addition, time-killing assay demonstrated a concentration and time-dependent bactericidal effect. At 0.35 mg/ml of aqueous extract, colony counts of S. aureus statistically significantly decreased from 115 at 2 hours to 0 at 24 hours (rs = -1.000, p-value < 0.001). At 0.4 mg/ml, bacterial colony elimination occurred rapidly, with colony counts reduced significantly to 0 at 8 hours (rs = -0.975, p-value = 0.02). These findings suggest that the aqueous Indian almond leaf extract may serve as a natural alternative to reduce antibiotic use against S. aureus infection. |
