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Anti-inflammatory, wound healing and anti-adherence activity of Clinacanthus nutans and its constituent |
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| รหัสดีโอไอ | |
| Title | Anti-inflammatory, wound healing and anti-adherence activity of Clinacanthus nutans and its constituent |
| Creator | Moehamad Orliando Roeslan |
| Contributor | Sitthichai Khunthongkaew, Advisor |
| Publisher | Thammasat University |
| Publication Year | 2560 |
| Keyword | Clinacanthus nutans, Purpurin-18 phytyl ester, Anti-inflammatory, Cell migration, Anti-adherence activities |
| Abstract | Clinacanthus nutans (C. nutans) has been known to have antiinflammatory activity, however the active compound of anti-inflammatory is still unknown. The aim of this study is to identify bioactive compound from C. nutans that responsible for anti-inflammatory, cell migration, and anti-adherence activities. Materials and methods: The isolation of pure compound from the extract of C. nutans was performed by chromatographic techniques and bioassay-guided fractionation. The pure isolated compound was characterized by spectroscopic data. The study included cytotoxicity assay, nitric oxide assay, wound scratch assay, adherence assay, and visualization of biofilm by confocal laser scanning microscopy. Results: The results showed that the chloroform extract from C. nutans and its pure isolated compound namely purpurin-18 phytyl ester significantly inhibited LPSinduced NO production at concentration 100 μg/mL for the chloroform extract, and 10 μg/mL for the isolated compound (p<0.05), significantly induced migration of gingival fibroblast at concentration 10 μg/mL (p<0.05), and significantly inhibited the adherence of S. mutans at concentration 12.5-1000 μg/mL for the chloroform extract, and 25 μg/mL for the isolated compound (p<0.05). Conclusion: These findings suggest that purpurin-18 phytyl ester may be the bioactive compound from C. nutans that responsible for anti-inflammatory, cell migration, and anti-adherence activities. |
