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Identification and transcriptional analysis of the metal tolerance protein (MTP) gene family in cassava under zinc deficiency |
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| รหัสดีโอไอ | |
| Title | Identification and transcriptional analysis of the metal tolerance protein (MTP) gene family in cassava under zinc deficiency |
| Creator | Nimnara Yookongkaew |
| Contributor | Natlita Payap, Triwarat Rujikiadtichok |
| Publisher | Mahasarakham University |
| Publication Year | 2564 |
| Keyword | zinc, zinc deficiency, cassava, metal tolerance proteins, gene expression |
| Abstract | Available of zinc in cassava plants is essential for plant developmental stages as well as crop production. This paper was explored the molecular mechanism of zinc transport in cassava plants. Zinc deficient symptom was found in upper cassava leaves under zinc deficiency and identified the metal tolerance proteins (MTPs) family in cassava genome. MTPs are a metal cation efflux transporter that participated in zinc homeostasis. Computational analysis showed 12 MeMTP members in cassava genome with exons ranging from 1-12. Most of the MTP proteins were predicted to localize in plasma membrane and tonoplast except MeMTP4 and MeMTP8, which localized in ER membrane. Functional annotation verified that MeMTPs were cation efflux proteins/zinc transporters belonging to cation diffusion facilitator superfamily (CDF). Most of the cis-acting elements in MeMTP promoters were phytohormone responsive. TGA-element was identified in MeMTP2, MeMTP5-7 and MeMTP10 promoters, indicating its role in auxin regulation. MeMTP proteins were divided into three groups according to phylogenetic relationship. Moreover, RNA expression of 6 candidate MeMTP genes including MeMTP1-5 and MeMTP12 was evaluated under zinc deficiency. MeMTP1 was up-regulated in roots and leaves under zinc deficiency whereas the expression of MeMTP2, MeMTP4 and MeMTP12 were tissue-specific. These findings will provide an important foundation of the MTPs in zinc homeostasis mechanism of cassava plants. |
| Language | English |
| URL Website | https://techno2.msu.ac.th/icofab2021/ |
| Website title | The 8th International Conference on Food Agriculture & Biotechnology |
