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METHOD VALIDATION FOR QUANTITATIVE DETERMINATION OF GALLIC ACID FROM ACACIA CONCINNA (WILLD.) D.C. |
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| รหัสดีโอไอ | |
| Title | METHOD VALIDATION FOR QUANTITATIVE DETERMINATION OF GALLIC ACID FROM ACACIA CONCINNA (WILLD.) D.C. |
| Creator | PATTRA AHMADI PIRSHAHID |
| Contributor | CHIRAMET AURANWIWAT, WIRIYAPORN SUMSAKUL, SINEE SIRICOON, WARAPORN SORNDECH |
| Publisher | Faculty of Technology, Mahasarakham University |
| Publication Year | 2563 |
| Keyword | Gallic acid, Acacia concinna, Method validation |
| Abstract | Gallic acid is one of the most antioxidative components found in Acacia genus. Acacia concinna (Willd.) D.C. belongs to Acacia genus and Fabaceae family, this genus has been used as traditional medicine. The development and validation of RP-HPLC method to determine gallic acid from the ethanolic extract of Acacia concinna. The chromatographic condition was performed on Symmetry Shield RP18 (4.6 x 250 mm) with ACN and 0.5% H3PO4 as mobile phases, flow rate 1.0 mL/min and detection at 272 nm. The quantitative results using external standard, standard gallic acid showed the linear range between 5-50 ?g/mL while LOD and LOQ exhibited the values at 0.87 and 2.65 ?g/mL, respectively. The accuracy and precision also examined. In the real sample, the ethanolic extract of A. concinna consisted of gallic acid 1.042 %w/w. |
| Language | EN |
| URL Website | https://techno2.msu.ac.th/icofab/welcome/ |
| Website title | The 7th International Conference on Food Agriculture & Biotechnology(ICoFAB 2020) |
