|
Utilization of sugarcane syrup and non-lysed dried spent brewer's yeast for cost effective succinate production by Actinobacillus succinogenes |
|---|---|
| รหัสดีโอไอ | |
| Title | Utilization of sugarcane syrup and non-lysed dried spent brewer's yeast for cost effective succinate production by Actinobacillus succinogenes |
| Creator | Apichai Sawisit |
| Contributor | Surawee Jampatesh, Sirima Suvarnakuta Jantama, Thipwarin Rimlumduan, Sukanya Lapkratok, Rawintra Eamrat, Sukanya Mingyai, Kaemwich Jantama |
| Publisher | Mahasarakham University |
| Publication Year | 2561 |
| Keyword | Succinate, sugarcane syrup, dried spent brewer's yeast, Actinobacillus succinogenes, CO2 |
| Abstract | In this study, non-lysed dried spent brewer's yeast (DSB) was evaluated as a low-cost nitrogen source for succinate production by Actinobacillus succinogenes 130ZT using sugarcane syrup as an inexpensive source of carbon. The results indicated that the DSB could promote the succinate production efficiency. Succinate concentration at 8.32 g/L with a yield of 0.82 (g/g glucose) from 15 g/L initial glucose that were supplemented with 30 g/L of DSB were achieved. This result was comparable to that obtained using commercial yeast extract at 5 g/L in which succinate concentration at 8.37 g/L was obtained. The highest yield and productivity of succinate at 0.74 g/g glucose and 0.53 g/L/h in glucose-based medium were obtained, respectively by controlling pH at 6.8 and continuously supplying external CO2. The optimized concentration of sugarcane syrup was observed at 10% (w/v) in which the succinate concentration at 35.75 g/L with a yield of 0.76 (g/g glucose) were attained. The present study suggested that DSB and sugarcane syrup could be used as nitrogen and carbon sources for industrial succinate production. |
| Language | English |
