Butanol production from molasses by Clostridium beijerinckii TISTR 1461 under different yeast extract concentration conditions
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Title Butanol production from molasses by Clostridium beijerinckii TISTR 1461 under different yeast extract concentration conditions
Creator Kanlayani Charoensopharat
Contributor Kitipong Wechgama
Publisher Mahasarakham University
Publication Year 2561
Keyword Clostridium beijerinckii TISTR 1461, butanol, molasses, yeast extract
Abstract This study aimed to investigate the effect of yeast extract concentrations (1-5 g l-1) for butanol production from sugarcane molasses by Clostridium beijerinckii TISTR 1461. The molasses without nutrient supplementation containing 60 g l-1 of total sugar and varied yeast extract concentrations at 1-5 g l-1 were used as butanol production media. The fermentation was carried out in a 1-L bottle with a working volume of 750 ml at 37 ?C and an initial pH of 6.5 under anaerobic condition. The results showed that butanol and ABE concentration increased with increasing of yeast extract concentration at 1-3 g l-1. However, the butanol concentration was slightly different at the yeast extract concentration between 4 and 5 g l-1 (10.48 and 10.39 g l-1) in the media. The highest butanol and ABE concentrations were obtained at 10.57 g l-1, and 14.73 g l-1, respectively when adding 3 g l-1 yeast extract. However, the results also showed the butanol yield and butanol productivity at 0.33 and 0.22 g l-1 h-1, respectively. This condition enhanced the butanol concentration, yield and productivity when compared with the control medium.
Language English
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