|
Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.) |
|---|---|
| รหัสดีโอไอ | |
| Title | Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.) |
| Creator | H T Dong |
| Contributor | P Khunrae, T Rattanarojpong, S Senapin |
| Publisher | Mahasarakham University |
| Publication Year | 2560 |
| Keyword | 16s rDNA A jandaei A varonii A hydrophila |
| Abstract | Motile Aeromonas species were known as a major cause of fish disease. Aeromonas species are commonly bacteria in aquatic environment. A.hydrophila is known as a major etiological agent of enteric pathogen in fish and aquaculture animal and identified as an danger species in Thailand for a long times while other species was overlooked. Here, we identified two isolates of Non- A. hydrophila mainly recovered from diseased Nile Tilapia exhibiting histopathology resemble to that A.hydrophila infected fish from commercial farm. These 2 stains were identified as A.jandaei and A.varonii base on 16s rDNA and phenotypic feature, respectively. From the challenging experiment, it revealed that challenging the fish with 3.7x106 CFU of A.jandaei and 2.9x106 CFU of A. varonii caused the mortality rate of 100% after challenging 24 h without any clinical signs. From the histological examination of death fish revealed the hemorrhagic of internal organ after challenging. The mortality rate of the challenged fish was reduced according to the dose of bacterial challenging. The survival challenged fish could resist to these two bacteria with the dramatic reduction of the mortality rate to 0%-12% despite challenging with 3.7x106 CFU of A. jandaei and 8.9x106 CFU of A. varonii |