Effect of hydrolyzed Cordyceps militaris on probiotic growth
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Title Effect of hydrolyzed Cordyceps militaris on probiotic growth
Creator Surachai Rattanasuk Tanyarat Pruemprom
Contributor Department of Science and Technology Faculty of Liberal Arts and Science Roi Et Rajabhat University Roi Et Thailand
Publisher Mahasarakham University
Publication Year 2560
Keyword Lactobacillus acidophilus TISTR 1338 Cordyceps militaris probiotic growth
Abstract Galactomannan is a polysaccharide found in Cordyceps militaris which is useful for mannooligosaccharide production. This research was aimed to study the effect of hydrolyzed C. militaris on probiotic growth. C. militaris was hydrolyzed by Bacillus methylotrophicus KS1, at 37 oC, 150 rpm for 7 days and reducing sugar was determined. The supernatant of hydrolyzed C. militaris was added into de Man, Rogosa and Sharpe MRS) medium containing each probiotic (Lactobacillus plantarum TISTR 543 or Lactobacillus casei TISTR 390 or Lactobacillus acidophilus TISTR 1338) in 96-well culture plate and hydrolyzed copra meal was used as a positive control in triplicate. Culture plates were incubated for 48 hr. The optical density (600 nm) of probiotic was measured at before and after incubation. The result demonstrated that hydrolyzed C. militaris can be promoted all probiotics but the maximum promoting was observed in L. acidophilus TISTR 1338.
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