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Isolation of thermophilic mannanase-producing bacteria |
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| รหัสดีโอไอ | |
| Title | Isolation of thermophilic mannanase-producing bacteria |
| Creator | Surachai Rattanasuk |
| Contributor | Roi Et Rajabhat University |
| Publisher | Mahasarakham University |
| Publication Year | 2559 |
| Keyword | Mannanase, Bacillus, Thermophilic |
| Abstract | The aim of this research was to isolate the mannanolytic thermophilic bacterium useful for mannooligosaccharide (MOS) production. Thermophilic mannanase-producing bacteria were isolated from 5 soil samples collected from Roi Et Rajabhat University. The bacteria were cultured using Luria-Bertani (LB) medium containing 1% locust bean gum (LBG) and incubated at 60 oC for 1 hr before continued culturing at 37 oC for 48 hr. The mannanase activity was screened on LB agar containing 1% LBG stained with iodine solution. The colonies that showed clear zone were selected. The isolates were cultured using LB broth containing LBG at 60 oC for 72 hr. One isolates were survived and exhibited the high amount of mannanase activities were selected and named KS1. Mannanase activity at 50-100 oC was measured using DNS method using LBG (pH 7.0) as mannan source and cultured broth as crude mannanase. The results indicated that at 60 oC showed the highest mannanase activity at 4.19U/ml. The thermostability of crude mannanase at 50 oC was 4.71. For bacterial identification using partial 16S rRNA indicated that KS1 was identity with Bacillus sp. at 98.6%. |
