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Isolation and characterization of thermophilic mannanase-producing bacteria isolated from soil |
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| รหัสดีโอไอ | |
| Title | Isolation and characterization of thermophilic mannanase-producing bacteria isolated from soil |
| Creator | Surachai Rattanasuk |
| Contributor | Roi Et Rajabhat University |
| Publisher | Mahasarakham University |
| Publication Year | 2559 |
| Keyword | Mannanase, Bacillus, Thermophilic |
| Abstract | The aim of this research was to isolate the mannanolytic thermophilic bacterium useful for mannooligosaccharide (MOS) production. Thermophilic mannanase-producing bacteria were isolated from 5 soil samples collected from Roi Et Rajabhat University. The bacteria were cultured using Luria-Bertani (LB) medium containing 1% locust bean gum (LBG) and incubated at 60 oC for 48 hours. The mannanase activity was screened on LB agar containing 1% LBG stained with iodine solution. The colonies that showed clear zone were selected. The isolates were cultured using LB broth containing LBG at 60 oC for 72 hr. Two isolates were survived and exhibited the high amount of mannanase activities were selected and named KS2 and KS6. Mannanase activity at 50-100 oC was measured using dinitrosalicylic acid method using LBG (pH 7.0) as mannan source and cultured broth as crude mannanase. The results indicated that at 60 oC, KS2 and KS6 showed the mannanase activity at 4.25 and 4.02 U/ml, respectively. The thermostable of crude mannanase activity of both at 50 oC were 3.72 and 3.96 U/ml. According to the morphological examination and 16S rRNA gene sequencing, both isolates KS2 and KS6 were identified as Bacillus amyloliquefaciens subsp. plantarum. |
