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Alpha-amylase inhibitory activity from crude protein extract of Thai rice seedsOryza sativavariety Hawm Tung and its partial protein purification |
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| รหัสดีโอไอ | |
| Title | Alpha-amylase inhibitory activity from crude protein extract of Thai rice seedsOryza sativavariety Hawm Tung and its partial protein purification |
| Creator | Anupong Joompang |
| Contributor | Arunta Samankul, Saranyu Khammaung, Rakrudee Sarnthima |
| Publisher | Mahasarakham University |
| Publication Year | 2558 |
| Keyword | alpha-amylase inhibitor protein, porcine pancreas alpha-amylase, Thai rice seeds |
| Abstract | This study aimed to characterize and partial purify ?-amylase inhibitor protein of crude extract from Thai rice seeds (Oryza sativa var. Hawm Tung). The result showed that the crude extract could inhibit porcine pancreas ?-amylase activity. The inhibitor was found to be heat stable up to 85 ?C and had two pH optima of 5 and 7. The crude extract could inhibit ?-amylase activity on gel as determined by native-PAGE. In partial purification, the crude extract was salt fractionated and subsequence separated by Q-sepharose column. Both bound and unbound protein fractions from Q-sepharose column contained the inhibitory activity. SDS-PAGE revealed that all of Q-sepharose fractions contained major proteins with molecular weight around 14-16 kDa. This research revealed the potential research and development of protein from Thai rice grain (Oryza sativa var. Hawm Tung) as food supplement product for blood sugar and weight loss control. |
