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Study the Best Support for Hydrolysis of Tiger Grass |
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| รหัสดีโอไอ | |
| Title | Study the Best Support for Hydrolysis of Tiger Grass |
| Creator | Kurizara Sayamnikorn |
| Contributor | Sujitra Wongkasemjit, Apanee Luengnaruemitchai, Thanyalak Chaisuwan |
| Publisher | Chulalongkorn University |
| Publication Year | 2560 |
| Keyword | Hydrolysis, Ethanol fuel industry -- Waste minimization, Trichoderma reesei, การแยกสลายด้วยน้ำ, อุตสาหกรรมเชื้อเพลิงเอทานอล -- การลดปริมาณของเสีย |
| Abstract | Bioethanol, one of the outstanding biofuels, is not only a renewable bio-based resource, but also interesting to research for improving better properties of the enzyme needed in the hydrolysis process. The purpose of this work is to study how to reuse the enzyme used in the hydrolysis step for bioethanol production by immobilizing Trichoderma reesei (T.reesei) on various silica supports, namely, SBA-15, TUD-1, and MCM-48. The amount of the adsorbed enzymes was determined by UV-visible spectrophotometry. Among those studied supports, SBA-15 showed 100% enzymatic adsorption on the support owing to its larger pore diameter of 6.14 nm, which is large enough to accommodate T.reesei enzyme molecules inside the pore channel. Various parameters, viz. temperature, pH, time, and amount of the support for optimizing the immobilized enzyme were investigated. The immobilized T.reesei on SBA-15 support was characterized by N2 adsorption-desorption. The amount of monomeric sugar after the hydrolysis process was measured by high performance liquid chromatography (HPLC). |
| URL Website | cuir.car.chula.ac.th |
