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α-glucosidase inhibitors from java tea Orthosiphon aristatus Miq |
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| รหัสดีโอไอ | |
| Title | α-glucosidase inhibitors from java tea Orthosiphon aristatus Miq |
| Creator | Doungkamon Toumsuk |
| Contributor | Preecha Phuwapraisirisan |
| Publisher | Chulalongkorn University |
| Publication Year | 2557 |
| Keyword | Diabetes -- Treatment, Orthosiphon Aristatus (Blume) Miq., Hyperglycemia, หญ้าหนวดแมว, เบาหวาน -- การรักษา, น้ำตาลในเลือดสูง |
| Abstract | Diabetes is a metabolic disorder where the body fails to utilize the ingested glucose properly. Type 2 diabetes is characterized by insulin resistance, which may be combined with relatively reduced insulin secretion. An effective strategy for type 2 diabetes therapy is to suppress glucose level through inhibition of intestinal α-glucosidase. Orthosiphon aristatus (Blume) Miq. (Java tea) known in Thai as “Ya-Nuad-Meo” have been recorded for in vivo hyperglycemia. In our research, crude extracts from leaves and twigs of O. aristatus prepared by two different extraction methods-decoction and maceration were screened for a-glucosidase inhibition. Apparently, the leave extract prepared by decoction with hot water showed more potent inhibition. Bioassay-guided fractionation of the aqueous extract from leaves led to the isolation of methyl caffeate (1), 3,4-dihydroxy benzaldehyde (2), methyl rosmarinate (3) and rosmarinic acid (4). The isolated compounds inhibited maltase and sucrase with IC50 values in the range of 0.061-0.738 mM, which are equipotent to standard antidiabetic drug acarbose. Furthermore, the kinetic investigation revealed that maltase was inhibited by methyl rosmarinate and rosmarinic acid through mixed-type whereas sucrase was inhibited through mixed-type and competitive manners. |
| URL Website | cuir.car.chula.ac.th |
