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Effects of artonin e on lung cancer cell anoikis |
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| รหัสดีโอไอ | |
| Title | Effects of artonin e on lung cancer cell anoikis |
| Creator | Ekkarat Wongpankam |
| Contributor | Pithi Chanvorachote |
| Publisher | Chulalongkorn University |
| Publication Year | 2555 |
| Keyword | Lungs -- Cancer, Cancer cells, ปอด -- มะเร็ง, เซลล์มะเร็ง |
| Abstract | Anoikisis apoptosis that is induced by loss of adhesion. Anoikisis recognized as a key inhibitory process of cancer metastasis. Because lung cancer cells possess an ability to resist anoikisresulting in a high rate of metastasis and death, the present study aimed to investigate a possible anoikis-sensitizing effect of artonin E (AE). AE was extracted from bark of Artocarpusgomezianus. Anoikis sensitization of AE was investigated in H460 human lung cancer cells. The viable cells were measured by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and level of anoikis-related proteins was determined by western blot analysis. AE was shown to enhance H460 cell anoikisin a concentration-dependent manner. We investigated the underlying mechanism of AE and found that AE sensitized the cells by down-regulating antiapoptotic myeloid leukemia cell sequence 1 (Mcl-1) protein but had no significant effect on other proteins including Bcl-2, Bcl-2-associated X protein (Bax), cav-1 and p53. Furthermore, anoikis sensitization of AE was consistently observed in A549 and H292 lung cancer cells. The present study demonstrates a novel activity of AE on lung cancer cell anoikis for the first time which might lead to the development of a new strategy for lung cancer therapy. |
| URL Website | cuir.car.chula.ac.th |
