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Genetics of antimicrobial resistance in Vibrio species isolated from farmed marine shrimps in Thailand |
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| รหัสดีโอไอ | |
| Title | Genetics of antimicrobial resistance in Vibrio species isolated from farmed marine shrimps in Thailand |
| Creator | Sirikorn Kitiyodom |
| Contributor | Rungtip Chuanchuen, Janenuj Wongtavatchai |
| Publisher | Chulalongkorn University |
| Publication Year | 2551 |
| Keyword | Drug resistance, Vibrio, Vibrio cholerae, Vibrio vulnificus, Shrimps -- Thailand |
| Abstract | A total of 83 Vibrio isolates from farmed marine shrimps, comprising V. parahaemolyticus (n=26), V. cholera (n=8), V.fluvalis (n=23) and V. vulnificus (n=16) were included in this study. Susceptibilities to 10 antimicrobials were determined. The occurrence and characteristics of class1, 2 and 3 integrons were investigated. The presence of tet(K), tet(L), tet(M), tet(O) and tet(S) genes were examined. Seventeen isolates resistant to ciprofloxacin and/or enrofloxacin were examined for mutations the Quinolone Resistance Determinig Regions (QRDRs) of gyrA and parC genes. Seventy-four isolates (89%) were resistant to at least one antibiotic and 17 isolates (21%) were multidrug-resistant. Most of the Vibrio isolates (62%) were resistant to ampicillin and the most common resistance pattern was the AMP-SMX-TMP (4.82%). As class 1 integrons were identified in 6%, no class 2 and 3 intrgrons were detected. Only one class 1 integrons in a V. cholera isolate carried gene cassettes. Nucleotide sequrncing analysis revealed that the inserted gene cassette was the partial rumA gene. All of class 1 integrons did not harbor the typical 3’conserved segment. None of the isoloates possessed tet genes tested. As Ser-83-lle substitution in GyrA was the major mutation identified in the fluoroquinolone-resistant isolates (59%), no mutation in ParC was observed. The results warranted further studies to investigate other mechanisms underlying resistance to antibiotics in the Vibrio isolates from farmed marine shrimps. |
| URL Website | cuir.car.chula.ac.th |
