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The mechanism of TGF-beta 1-induced-MMP-9 expression in head and neck squamous cell carcinoma |
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| รหัสดีโอไอ | |
| Title | The mechanism of TGF-beta 1-induced-MMP-9 expression in head and neck squamous cell carcinoma |
| Creator | Sariya Nuchanardpanit |
| Contributor | Prasit Pavasant, Atiphan Pimkhaokham, Neeracha Sanchavanakit |
| Publisher | Chulalongkorn University |
| Publication Year | 2550 |
| Keyword | Cancer, Carcinoma, Squamous Cell, Head and Neck Neoplasms, Matrix Metalloproteinase 9, Transforming Growth Factor Beta 1 |
| Abstract | Matrix metalloproteinase-9 (MMP-9) plays roles in cancer progression by degrading the extracellular matrix and basement membrane. Many growth factors including Transforming growth factor-beta1 (TGF-beta1) could induce MMP-9 expression. We demonstrated that TGF-beta1 induced MMP-9 mRNA and protein in human head and neck squamous cell carcinoma cell lines. Application of TGF-beta receptor type I inhibitor (SB505124) reduced the MMP-9 expression markedly. Whilst, inhibitor of Myosin light chain kinase (MLCK) could reduce the level of secreted MMP-9 in both the supernatants and cell lysate but not the level of MMP-9 mRNA. These suggested that MLCK might regulate MMP-9 expression post-transcriptionally. Application of SB505124 and siRNA Smad2/3 reduced the phosphorylation of myosin light chain (MLC) suggested that MLC is downstream to T-betaRI/Smad2/3 signaling pathway. In conclusion, these results describe a novel mechanism for the potentiation of TGF-beta1 signaling to induce MMP-9 expression via Smad and MLCK. |
| URL Website | cuir.car.chula.ac.th |
