|
Analysis of morphometric and genetic variation of small dwarf honey bees Apis andreniformis Smith, 1858 in Thailand |
|---|---|
| รหัสดีโอไอ | |
| Title | Analysis of morphometric and genetic variation of small dwarf honey bees Apis andreniformis Smith, 1858 in Thailand |
| Creator | Atsalek Rattanawannee |
| Contributor | Siriwat Wongsiri, Chanpen Chanchao |
| Publisher | Chulalongkorn University |
| Publication Year | 2549 |
| Keyword | Honeybee -- Thailand, Variation (Biology) |
| Abstract | Small dwarf honey bee, Apis andreniformis, is one of native Thai honey bees. Less data on morphometric and genetic variation of this species have been reported. In this investigaion, thirty colonies of A. andreniformis were collected for morphometric analysis and 37 colonies were collected for genetic analysis. For morphometric analysis, 24 characters of worker bees were measured and analysis. By using colony means for the 1st factor analysis, 20 out of 24 morphometric characters were selected as new variable. For the 2nd analysis, 20 morphometric characters could be grouped into 4 new factors. Due to graph plotting of factor scores, bees from Thailand and from Tenom, Malaysia belong into one group. In addition, a dendrogram generated from cluster analysis supports that bees from Thailand and Tenom, Malyaysia are clumped into one group. However, result on linear regression analysis of factor scores against latitude and longitude shows clinal patterns in morphometric characters of A. andreniformis in Thailand. The body size of bees from the south to the north increase but decreased in bees from the west to the east. Genetic variation was determined into 2 means. First, genetic variation was analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). After amplification of cytochrome oxidase subunit b (cytb), products of 520 bp were restricted by DraI and AluI. Genetic variation was observed. Six haplotypes were found after AluI digestion while 3 haplotypes were found after DraI digestion. Second, PCR products amplified by cytb were sequenced. Based on nucleoide analysis, DNA polymorphism among bees from mainland of Thailand is lower that from Phuket Island and Chiang Mai. Phylogenetic trees were constructed by Neighbor-joining and UPGMA programs. Two different groups of A andreniformis of Thailand are obtained from both trees. Bees in group A are from mainland while bees in group B are from Phuket Island and Chiang Mai. |
| ISBN | 9741420188 |
| URL Website | cuir.car.chula.ac.th |
