Chemical composition, antioxidant and antibacterial activities ofultrasound-assisted extract of Annona squamosa L. leaves
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Creator 1. Pornpun Siramon
2. Thitima Wongsheree
Title Chemical composition, antioxidant and antibacterial activities ofultrasound-assisted extract of Annona squamosa L. leaves
Publisher Research and Development Office, Prince of Songkla University
Publication Year 2022
Journal Title Songklanakarin Journal of Science an Technology (SJST)
Journal Vol. 44
Journal No. 1
Page no. 40-44
Keyword Annona squamosa L., antibacterial activity, antioxidant activity, chemical composition, ultrasound-assisted extraction (UAE)
URL Website https://rdo.psu.ac.th/sjst/index.php
ISSN 0125-3395
Abstract The bioactive compounds were extracted from Annona squamosa leaves using ultrasound-assisted extraction (UAE)and the optimal extraction conditions were determined. It was found that the extraction of the sample with 50% (aq) of ethanol at50 ยบC for 60 minutes provided the highest crude extract yield of 30.50% on a dry basis. Further, this condition gave a totalphenolic content of 307.67 ยตg GAE/g DW and total flavonoid content of 16,893.92 ยตg CE/g DW. The crude extract obtainedfrom this condition gave the best results for antioxidant activities with the IC50 values of 947.99 and 620.89 ยตg/mL by DPPH andABTS assays, respectively. The chemical composition of the crude extract was analysed by LC-ESI-MS, Rutin andNorisocorydine were detected. Finally, the antibacterial properties of the crude extract were tested against three humanpathogenic bacterial strains. Results revealed that the crude extract exhibited antibacterial activities against all tested strains.
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