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Protective effect of Perilla leaf extract against ROS formationand inflammation induced by TNF-ฮฑ in A549human lung carcinoma cell line |
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| รหัสดีโอไอ | |
| Creator | 1. Wanisa Punfa 2. Chakkrit Khanaree 3. Komsak Pintha 4. Orada Chumphukam 5. Maitree Suttajit 6. Payungsak Tantipaiboonwong |
| Title | Protective effect of Perilla leaf extract against ROS formationand inflammation induced by TNF-ฮฑ in A549human lung carcinoma cell line |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2022 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 2 |
| Page no. | 361-369 |
| Keyword | Perilla frutescens, TNF-๏ก, antioxidant, anti-inflammatory, A549 cancer cells |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | Perilla frutescens is an herb traditionally used to treat chronic diseases owing to its antioxidant and anti-inflammatoryproperties. This study aimed to determine the antioxidant and anti-inflammatory effects of perilla leaf extract (PLE) in TNF-๏ก-treated A549 human lung carcinoma cell line. Perilla frutescens leaves were extracted with 70% ethanol, and the phenolic,flavonoid, and rosmarinic acid contents were determined. Cytotoxicity of PLE was assessed using the MTT assay. Theantioxidant activity and intracellular ROS production in TNF-๏ก-treated A549 cells were evaluated. Furthermore, mRNAexpression of pro-inflammatory mediators was assessed by RT-qPCR. These results showed that PLE contained phenolic acid,flavonoids, and rosmarinic acid and had no cytotoxicity. PLE exhibits antioxidant activity through a reduction in intracellularROS production in TNF-๏ก-treated A549 cells. Moreover, PLE showed significantly decreased mRNA expression of IL-1๏ข, IL-8,TNF-๏ก and COX-2. Thus, PLE plays an important role as an antioxidant and anti-inflammatory agent for the prevention of ROSformation and inflammation in A549 cells. |
