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Effect of additives on shelf-life of suspension of photosyntheticbacteria tested using full factorial experimental design |
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| รหัสดีโอไอ | |
| Creator | 1. Piyarat Boonsawang 2. Wasan Sanguanmanasak |
| Title | Effect of additives on shelf-life of suspension of photosyntheticbacteria tested using full factorial experimental design |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2022 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 1 |
| Page no. | 162-168 |
| Keyword | Ectothiorhodospira shaposhnikovii, additives, viable cell, full factorial experimental design |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | This study aimed to investigate the effects of additives, seeking to extend longevity of a viable cell count (>106CFU/mL) of Ectothiorhodospira shaposhnikovii during storage at room temperature (30ยฑ2oC). E. shaposhnikovii were culturedin a 1000L-fermenter until the viable cells reached 4.85ยฑ0.50ร106 CFU/mL. Afterward, samples were stored in 1L translucentcylindrical plastic bottles for 60 days. Effects of glycerine (0, 0.15%), Na2HPO4ยท2H2O (0, 0.5 g/L), and Na2CO3 (0, 0.5 g/L) onviable cells were investigated in a full factorial experimental design. Na2CO3 had significant effect and maintained viable cells at2.35ยฑ0.120ร106 CFU/mL (a survival rate of 48.4%) and at 1.15ยฑ0.057ร106 CFU/mL (a survival rate of 23.7%) after 45 and 60days, respectively. In contrast, glycerine negatively affected the viable cells of E. shaposhnikovii, reducing the counts to 0.037-0.255ร106and 0.028-0.128ร106 CFU/mL after 45 and 60 days, respectively. This indicates that Na2CO3 can be used as a lowcost additive to extend the storage shelf life of E. shaposhnikovii in commercial stage. |
