|
Role of GABA and its receptors in anti-adipogenesisin cultured adipocytes |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Kanittaporn Trisat 2. Saowalak Yomlar 3. Nanteetip Limpeanchob |
| Title | Role of GABA and its receptors in anti-adipogenesisin cultured adipocytes |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2020 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 5 |
| Page no. | 1053-1058 |
| Keyword | GABA, GABA receptor, adipogenesis, lipolysis, adipocyte |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Health benefits of GABA and GABA-enriched foods via anti-obesity activity have been demonstrated in animals.However, the functional roles of GABA especially as non-neurotransmitter in adipocytes have not been thoroughly clarified.Thus, this study aimed to assess the impact of GABA on adipogenesis and lipolysis using 3T3-L1 adipocytes. Oil Red O stainingwas used to assess lipid accumulation and glycerol release was determined as an indicator of lipolysis. The results showed thattreatment with GABA (100-500 ?M) during adipocyte maturation decreased lipid accumulation by ?20-30%. GABA clearlyinduced the release of glycerol in a dose-dependent manner within 4 h of treatment, and GABA at 500 ?M increased glycerolrelease about two-fold relative to control. GABA-mediated lipolysis depended on both GABA-A and GABA-B receptors as wellas on ?-adrenergic receptors. The findings suggest that GABA exhibits non-neuronal functions by promoting lipolysis and bysuppressing fat accumulation in adipocytes, which potentially support anti-obesity activity of GABA and GABA-enrichedproducts. |
