UV-induced mutagenesis in Volvariella volvaceato improve mushroom yield
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Creator 1. Eakaphun Bangyeekhun
2. Korapan Sawetsuwannakul
3. Urarux Romruen
Title UV-induced mutagenesis in Volvariella volvaceato improve mushroom yield
Publisher Research and Development Office, Prince of Songkla University
Publication Year 2020
Journal Title Songklanakarin Journal of Science and Technology
Journal Vol. 42
Journal No. 4
Page no. 910-916
Keyword mutagenesis, straw mushroom, UV, Volvariella volvacea, yield
URL Website https://rdo.psu.ac.th/sjstweb/index.php
ISSN 0125-3395
Abstract Low biological efficiency is a defect in straw mushroom production. Mycelia of the straw mushroom were exposed toUV radiation to induce mutagenesis and improve the strains of straw mushroom for a high yield. A UV exposure time thatyielded a survival rate of about 25% was applied for mutation induction. After UV treatment, 65 putative mutant strains wereobtained and seven fast-growing strains were selected for further analysis. Mushroom cultivation experiments revealed that thebiological efficiency of four of the new strains was approximately 30% higher than the parental strain. The radial mycelial colonygrowth rate was assessed on different media and under various temperature and pH conditions for physical characterization. Theresults indicated that the overall growth rates of the mutant strains were higher than the parental strain. Genetic characterizationby random amplification of polymorphic DNA analysis revealed that the genome had been altered in the mutant strains.
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