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Effect of a recombinant ribosomal protein L10a (rRpL10a)on mouse spermatogenesis |
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| รหัสดีโอไอ | |
| Creator | 1. Kunwadee Palasin 2. Walaiporn Makkapan 3. Monwadee Wonglapsuwan 4. Wilaiwan Chotigeat |
| Title | Effect of a recombinant ribosomal protein L10a (rRpL10a)on mouse spermatogenesis |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2019 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 5 |
| Page no. | 959-965 |
| Keyword | rRpL10a, spermatogenesis, testis, mice |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | This study attempted to clarify RpL10a function in testis using mouse testis as a model. The histological method ofmouse testis was used to determine the percentages of cell types in the spermatogenesis after being treated with a recombinantRpL10a protein (rRpL10a). The percentages of spermatid cells in the testis treated with 0.5, 1.0, 1.5, and 2.0 ?M of rRpL10aprotein were 40.78%, 49.65%, 45.03%, and 45.33%, respectively, while the untreated group was 21.72%. The spermatid cells ofthe experiment tissue were confirmed by WGA lectin histochemistry. Prm2 gene expression was moderately increased, whereasthe expression of Rhau gene decreased in rRpL10a treated testes compared to the untreated testis. Furthermore, increased cellproliferation in the testes treated with 1.0 ?M rRpL10a was revealed by an intense fluorescent signal of EdU (5-ethynyl-2?-deoxyuridine) staining. Altogether, the results indicated that the rRpL10a protein induced spermatogenesis in mice and may beapplicable for in vitro spermatogenesis induction in the future. |
