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Amino acid analysis of lipases from oil pollutant isolates:Cunninghamella verticillata and Geotrichum candidum |
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| รหัสดีโอไอ | |
| Creator | 1. Subash C. B. Gopinath 2. Periasamy Anbu 3. M. K. Md Arshad 4. Thangavel Lakshmipriya 5. Chun Hong Voon 6. Uda Hashim 7. Yarub Al-Douri 8. Marimuthu Citartan 9. Thean-Hock Tang 10. Yeng Chen |
| Title | Amino acid analysis of lipases from oil pollutant isolates:Cunninghamella verticillata and Geotrichum candidum |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 4 |
| Page no. | 878 |
| Keyword | amino acid, Cunninghamella verticillata, Geotrichum candidum, high performance liquid chromatography, lipase |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Lipase is an enzyme commonly used in food, dairy, and other industries. Fungal lipases are more commonly used dueto their secretion and easier production. Analyses of the amino acid composition of microbial lipases will hasten their potentialusage in industrial applications. In this study, the major amino acid compositions of lipases secreted by oil pollutant isolates(Cunninghamella verticillata and Geotrichum candidum) enriched with fatty substances were analyzed by high performanceliquid chromatography. Among eight major amino acids found in these lipases, histidine and ornithine were predominant. Lysinewas absent from lipase generated by C. verticillata, while glutamine was absent from that produced by G. candidum. Conversely,glutamic acid, asparagine, histidine and arginine were present in slightly higher amounts in G. candidum. However, a slightdecrease in aspartic acid and ornithine was observed in G. candidum. Analyses of the amino acids composition of lipase canpotentially facilitate to predict the nature of this enzyme. |
