Tuna viscera hydrolysate products prepared by different enzyme preparationsimprove the feed intake and growth of Asian seabass, Lates calcarifer,fed total fishmeal replacement diets
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Creator 1. Rutchanee Chotikachinda
2. Chutima Tantikitti
3. Soottawat Benjakul
4. Turid Rustad
Title Tuna viscera hydrolysate products prepared by different enzyme preparationsimprove the feed intake and growth of Asian seabass, Lates calcarifer,fed total fishmeal replacement diets
Publisher Research and Development Office, Prince of Songkla University
Publication Year 2018
Journal Title Songklanakarin Journal of Science and Technology
Journal Vol. 40
Journal No. 1
Page no. 167
Keyword tuna viscera, degree of hydrolysis, inclusion level, feeding stimulant, Asian seabass
URL Website http://rdo.psu.ac.th/sjstweb/index.php
ISSN 0125-3395
Abstract Protein hydrolysate products prepared using different enzymes at different degrees of hydrolysis (DH) may havedifferent qualities when applied in a fish diet. In this study, hydrolysis enzymes, including endoproteinase from Bacilluslicheniformis (A), endoproteinase and exopeptidase from Aspergillus oryzae (F), and endoproteinase from B. amyloliquefaciens(N) were used to prepare tuna viscera hydrolysate products (TVHPs) at low (1) and high (2) DH (40 and 60%, respectively) forsupplementation in fishmeal free diets for Asian seabass. The TVHPs were tested in a feeding trial composed of ten diets: A1-and F1-TVHP (50g/kg), F2- and A2-TVHP (25 g/kg) of diet, and N-hydrolysed TVHP (either low or high DH) at 25, 50, and 75g/kg of diet. A control poultry by-product based diet and a fishmeal reference diet were included. Feed intake of fish fed theTVHP supplemented diets improved by 25.4-41.0% which significantly increased growth performance compared to the controlgroups (P<0.05). Growth response was better with diets containing 60% DH-TVHP suggesting that using a TVHP with a higherDH makes it possible to reduce the inclusion rate while still obtaining a performance benefit. In conclusion, the inclusion levelsof TVHPs at 25-75 g/kg of diet could be suitable as a feeding stimulant in fishmeal free diets for this carnivorous fish.
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