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Microbial diversity analysis of thermophilic hydrogen-producing consortia from hot spring in the south of Thailand and evaluate cashew apple juice as a substrate |
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| รหัสดีโอไอ | |
| Creator | 1. Atipan Saimmai 2. Wiboon Riansa-ngawong 3. Suppasil Maneerat 4. Paweena Dikit |
| Title | Microbial diversity analysis of thermophilic hydrogen-producing consortia from hot spring in the south of Thailand and evaluate cashew apple juice as a substrate |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2017 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 6 |
| Page no. | 803 |
| Keyword | biohydrogen,cashew apple juice,hot springs,microbial community analysis,thermophilic consortia |
| ISSN | 0125-3395 |
| Abstract | This study aims to develop thermophilic hydrogen-producing consortia from ten sediment samples of hot springs insouthern Thailand by repeated batch cultivation at 60?C with fructose, glucose and combination of fructose and glucose. Thepromising hydrogen production potentials consortia were obtained from thermophilic enriched cultures and encoded as NST1consortia. NST1 consortia exhibited the maximum hydrogen yields of 259 ml/g sugar consumed when fructose-glucose mixedwas used as a substrate. PCR amplified 16S rDNA product was separated by using denaturing gradient gel electrophoresis(DGGE) to identify the diversity of bacteria in NST1 consortia. The phyologenetic profile of the NST1 consortia showeda significant diversity in the microbial community, where major nucleotide sequences were affiliated to class Clostridia,followed by Bacteroidetes, Deltaproteobacteria, and Flavobacteria. Time course of hydrogen production revealed that NST1consortia gave the maximum hydrogen yield of 540 ml H2/g sugar consumed after 96 hrs of cultivation when 60% of cashewapple juice was used as a substrate. |
