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Improvement of drought tolerance in Thai rice cultivar RD6 through somaclonal variation |
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| รหัสดีโอไอ | |
| Creator | 1. Sumontip Bunnag 2. Anawat Suwanagul |
| Title | Improvement of drought tolerance in Thai rice cultivar RD6 through somaclonal variation |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2017 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 6 |
| Page no. | 723 |
| Keyword | drought tolerance,RD6,somaclonal variation,polyethylene glycol,mannitol |
| ISSN | 0125-3395 |
| Abstract | Thai rice cultivar RD6 was induced for drought tolerance through somaclonal variation. Nitsch and Nitsch (NN)medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 700 mg/l casein hydrolysate were employed forcallus induction and proliferation, whilst the same medium containing 0.5 mg/l naphthaleneacetic acid (NAA) combined with4 mg/l 6-benzylaminopurine (6-BA) was suitable for plant regeneration. Drought stress was stimulated by treatment withpolyethylene glycol (PEG, 0, 0.5, 1, 1.5 and 2% in v/v, MW6000) and/or mannitol (0,150, 300, 450 and 600 mM, MW182.17). Theresults showed the decreased survival percentage of calli exposed to the increasing PEG or mannitol concentration. Followingdrought treatment, surviving calli could develop into whole plants when grown on regeneration mediums. RAPD analysisemployed to determine the genetic relationship between the control plants and the drought-tolerant plants showed that thecontrol plants and the drought-tolerant plants were genetically different. |
