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Assessment of the bacterial community of soils contaminatedwith used lubricating oil by PCR-DGGE |
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| รหัสดีโอไอ | |
| Creator | 1. Naruemon Meeboon 2. Sireewan Kaewsuwan 3. Mary Beth Leigh 4. Suppasil Maneerat |
| Title | Assessment of the bacterial community of soils contaminatedwith used lubricating oil by PCR-DGGE |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2016 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 6 |
| Page no. | 667-674 |
| Keyword | enrichment cultures,microbial community,PCR-DGGE,used lubricating oil,soil bacteria |
| ISSN | 0125-3395 |
| Abstract | The diversity of indigenous bacteria in three soils contaminated with used lubricating oil (ULO) was determined andcompared using molecular analysis of bacteria cultured during the enrichment process. Sequencing analyses demonstratedthat the majority of the DGGE bands in enrichment cultures were affiliated with four phyla of the domain, Bacteria: ?, ?, ?-Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. Soil C had a higher ULO contamination level than soil A and B,which may explain why enrichment culture C had the greatest diversity of bacteria, but further studies would be needed todetermine whether ULO concentration results in higher diversity of ULO-degraders in soils. The diversity of ULO-degradersdetected in these three different soils suggests that biostimulation methods for increasing the activity of indigenous microorganismsmay be a viable approach to bioremediation, and that future studies to determine how to increase their activityin situ are warranted. |
