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Tumor necrosis factor alpha of teleosts:in silico characterization and homology modeling |
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| รหัสดีโอไอ | |
| Creator | 1. Tran Ngoc Tuan 2. Wang Gui-Tang 3. Pham Minh Duc |
| Title | Tumor necrosis factor alpha of teleosts:in silico characterization and homology modeling |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2016 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 5 |
| Page no. | 549-557 |
| Keyword | in silico,fish,TNF-? |
| ISSN | 0125-3395 |
| Abstract | Tumor necrosis factor alpha (TNF-?) is known to be crucial in many biological activities of organisms. In this study,physicochemical properties and modeling of TNF-? protein of fish was analyzed using in silico approach. TNF-? proteinsselected from fish species, including grass carp (Ctenopharyngodon idella), zebra fish (Danio rerio), Nile tilapia(Oreochromis niloticus), goldfish (Carassius auratus), and rainbow trout (Oncorhynchus mykiss) were used in this study.Physicochemical characteristics with molecular weight, theoretical isoelectric point, extinction coefficient, aliphatic index,instability index, total number of negatively charged residues and positively charged residues, and grand average of hydropathicitywere computed. All proteins were classified as transmembrane proteins. The "transmembrane region" and "TNF"domain were identified from protein sequences. The function prediction of proteins was also performed. Alpha helices andrandom coils were dominating in the secondary structure of the proteins. Three-dimensional structures were predicted andverified as good structures for the investigation of TNF-? of fish by online server validation. |
