รหัสดีโอไอ 10.14456/ijps.2018.17
Creator Waranya Chatuphonprasert
Title Effects of Nicotine, Rifampicin and Benzo[a]pyrene on the Expression of Cytochrome P450 in Human Hepatocellular Carcinoma Cells
Contributor Thanyalak Boonmaton, Kanokwan Jarukamjorn
Publisher Faculty of Pharmaceutical Sciences KKU MSU UBU
Publication Year 2018
Journal Title Isan Journal of Pharmaceutical Sciences
Journal Vol. 14
Journal No. 2
Page no. 94-104
URL Website https://tci-thaijo.org/index.php/IJPS
Website title Isan Journal ofPharmaceutical Sciences, IJPS
ISSN 19050852
Abstract Introduction: CYP1, CYP2 and CYP2E, members of cytochrome P450 enzymes (CYP), are mainly expressed in livers and response for several chemicals in daily life. Nicotine, a compound in cigarettes, is metabolized by CYP2A6 and CYP2A13. Benzo[a]pyrene (BaP), a carcinogen found in grilled food and smoke from engines, is metabolized by CYP1A2. BaP is also an inducer of several CYP isoforms including CYP1A2. Rifampicin, an anti-tuberculosis drug, has potency to induce several CYPs and causes clinical drug interactions. The objective of this study is to determine the effect of nicotine, rifampicin, and BaP on the expression of CYP1A2, CYP2A6, CYP2A13, and CYP2E1 in human hepatocellular carcinoma (HepG2) cells. Methods: HepG2 was treated with nicotine (1, 10, and 100 ?M), rifampicin (10 ?M), BaP (10 ?M), and 0.1% DMSO (as the control) for 24 h before total RNA extraction. After that, the expression of CYP1A2, CYP2A6, CYP2A13, and CYP2E1 mRNA was determined using RT-qPCR, compared to a reference gene, GAPDH. Results: Nicotine induced CYP2A6 and CYP2A13 in dose-dependent pattern while CYP1A2 was induced by low dose of nicotine (1 ?M), same as BaP. CYP2E1 was induced by high dose of nicotine (100 ?M) and BaP. Rifampicin significantly induced the expression of CYP2A13 and CYP2E1. Moreover, BaP induced the expression of CYP1A2 and CYP2E1, and interestingly induced CYP2A6 and CYP2A13 significantly. Only nicotine at the low dose induced arylhydrocarbon receptor (AhR). Conclusion: Nicotine induced the expression of CYP2A6, CYP2A13, CYP1A2, and CYP2E1 mRNAs, comparable to BaP, and the regulatory pathway of CYPs by nicotine might related to AhR, at least in part.
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Waranya Chatuphonprasert และผู้แต่งคนอื่นๆ. (2018) Effects of Nicotine, Rifampicin and Benzo[a]pyrene on the Expression of Cytochrome P450 in Human Hepatocellular Carcinoma Cells. Isan Journal of Pharmaceutical Sciences,14,94-104.
Waranya Chatuphonprasert และผู้แต่งคนอื่นๆ. "Effects of Nicotine, Rifampicin and Benzo[a]pyrene on the Expression of Cytochrome P450 in Human Hepatocellular Carcinoma Cells". Isan Journal of Pharmaceutical Sciences 14 (2018):94-104.
Waranya Chatuphonprasert และผู้แต่งคนอื่นๆ. Effects of Nicotine, Rifampicin and Benzo[a]pyrene on the Expression of Cytochrome P450 in Human Hepatocellular Carcinoma Cells. Faculty of Pharmaceutical Sciences KKU MSU UBU:ม.ป.ท. 2018.