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Discovery of Aspergillusmycoviruses and development of mycovirus-based vector for gene silencing in the human pathogenic fungus Aspergillus fumigatus |
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| รหัสดีโอไอ | |
| Creator | LakkhanaK. Wingfield |
| Title | Discovery of Aspergillusmycoviruses and development of mycovirus-based vector for gene silencing in the human pathogenic fungus Aspergillus fumigatus |
| Contributor | Jirawalun Atcharawiriyakul, Robert Coutts |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2564 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 26 |
| Journal No. | 3 |
| Page no. | 9 |
| Keyword | Aspergillus fumigatus, Mycovirus, RNA silencing, Virus vectorsilencing, Virus vector |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/252626 |
| ISSN | 2539-6293 |
| Abstract | A collection of Aspergillusenvironmental isolates was screened for the presence of mycoviruses. Out of 178 isolates, 5.1% contained double-stranded RNA (dsRNA) elements ranging in size from approximately 0.7 to 6.0 kbp. The dsRNAelements were identified as belongingto four different categoriescomprising non-segmented dsRNA, bipartite dsRNA, quadripartite dsRNA and uncharacterized mycoviruses. Aspergillus fumigatustetramycovirus-1 (AfuTmV-1) was exploited for development as a tool for silencing genes in the human pathogenic fungus A. fumigatus. Using a sequence of dsRNA2 belonging to the AfuTmV-1, a recombinant virus vector was engineered to generate the silencing vector AfuTmV-1-dsRNA2::ALB1 which carried a target ALB1/PKSPgene responsible for spore pigmentation. The engineered virus vector induced loss of conidial pigmentation inthe fungal host atdifferent levels and with low frequency. In addition, in vitrotranslation of mycovirus-based silencing vectors demonstrated that the engineered mycoviral vector was functionally transcribed and translated. |
