รหัสดีโอไอ 10.14456/apst.2021.59
Creator LakkhanaK. Wingfield
Title Discovery of Aspergillusmycoviruses and development of mycovirus-based vector for gene silencing in the human pathogenic fungus Aspergillus fumigatus
Contributor Jirawalun Atcharawiriyakul, Robert Coutts
Publisher Asia-Pacific Journal of Science and Technology
Publication Year 2021
Journal Title Asia-Pacific Journal of Science and Technology
Journal Vol. 26
Journal No. 3
Page no. 9
URL Website https://www.tci-thaijo.org/index.php/APST
Website title https://so01.tci-thaijo.org/index.php/APST/article/view/252626
ISSN 2539-6293
Abstract A collection of Aspergillusenvironmental isolates was screened for the presence of mycoviruses. Out of 178 isolates, 5.1% contained double-stranded RNA (dsRNA) elements ranging in size from approximately 0.7 to 6.0 kbp. The dsRNAelements were identified as belongingto four different categoriescomprising non-segmented dsRNA, bipartite dsRNA, quadripartite dsRNA and uncharacterized mycoviruses. Aspergillus fumigatustetramycovirus-1 (AfuTmV-1) was exploited for development as a tool for silencing genes in the human pathogenic fungus A. fumigatus. Using a sequence of dsRNA2 belonging to the AfuTmV-1, a recombinant virus vector was engineered to generate the silencing vector AfuTmV-1-dsRNA2::ALB1 which carried a target ALB1/PKSPgene responsible for spore pigmentation. The engineered virus vector induced loss of conidial pigmentation inthe fungal host atdifferent levels and with low frequency. In addition, in vitrotranslation of mycovirus-based silencing vectors demonstrated that the engineered mycoviral vector was functionally transcribed and translated.
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บรรณานุกรม

LakkhanaK. Wingfield และผู้แต่งคนอื่นๆ. (2021) Discovery of Aspergillusmycoviruses and development of mycovirus-based vector for gene silencing in the human pathogenic fungus Aspergillus fumigatus. Asia-Pacific Journal of Science and Technology, 26(3), 9.
LakkhanaK. Wingfield และผู้แต่งคนอื่นๆ. "Discovery of Aspergillusmycoviruses and development of mycovirus-based vector for gene silencing in the human pathogenic fungus Aspergillus fumigatus". Asia-Pacific Journal of Science and Technology 26 (2021):9.
LakkhanaK. Wingfield และผู้แต่งคนอื่นๆ. Discovery of Aspergillusmycoviruses and development of mycovirus-based vector for gene silencing in the human pathogenic fungus Aspergillus fumigatus. Asia-Pacific Journal of Science and Technology:ม.ป.ท. 2021.