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Anti-proliferation and Anti-migration of Schizophyllum commune extracts on Human Cholangiocarcinoma Cell Line |
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| รหัสดีโอไอ | |
| Creator | 1. Apaporn Menakongka 2. Siriluk Ruaengsritanyakij 3. Siritorn Sripayak 4. Tuangporn Suthiphongchai |
| Title | Anti-proliferation and Anti-migration of Schizophyllum commune extracts on Human Cholangiocarcinoma Cell Line |
| Publisher | Text and Journal Publication |
| Publication Year | 2562 |
| Journal Title | Vajira Medical Journal |
| Journal Vol. | 63 |
| Journal No. | 5 |
| Page no. | - |
| Keyword | Cholangiocarcinoma (CCA), Schizophyllum commune, mushroom extracts, anticancer |
| URL Website | https://tci-thaijo.org/index.php/VMED |
| Website title | Vajira Medical Journal (วชิรเวชสาร) |
| ISSN | 0125-1252 |
| Abstract | Objective: To investigate the possible anti-proliferation and anti-migration effect of the crude extracts from Schizophyllum commune against human cholangiocarcinoma cell line, KKU-M213. Methods: In this study, dried fruiting bodies of S. commune were extracted with 95% ethanol and water. Antiproliferative activity of the crude extracts from S. commune against human cholangiocarcinoma cell line, KKU-M213 was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, diphenyl tetrazolium bromide (MTT) assay. Wound healing assay was used for investigating the effects of the mushrooms crude extracts on KKU-M213 cell migration. Results: The percentage yields of S. commune ethanol and water extracts were 5.42 % and 7.78 %, respectively. The results of MTT assay showed that 200 ?g/ml of ethanol crude extracts significantly inhibited proliferation of KKU-M213 to 50% compared to untreated control. While hot water extracts of S. commune did not have any effects on KKU-M213 cell proliferation. Wound healing assay result showed that both hot water and ethanolic extracts of S. commune suppressed KKU-M213 cell migration. Conclusion: This is the first report showing the anti-proliferation and anti-migration activity of this mushroom against human cholangiocarcinoma. The active anticancer components of S. commune extracts will be further investigated. |
