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The Detection of Opisthorchis viverrini DNA in Bile Samples from Obstructive Jaundice Patients by Real-time PCR |
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| รหัสดีโอไอ | |
| Creator | 1. Nithikoon Aksorn 2. Sittiruk Roytrakul 3. Kawin Leelawat 4. Shinjiro Hamano 5. Sundusit Pengsa 6. Namthip Munponsri 7. Usa Lek-Uthai |
| Title | The Detection of Opisthorchis viverrini DNA in Bile Samples from Obstructive Jaundice Patients by Real-time PCR |
| Publisher | Text and Journal Publication |
| Publication Year | 2561 |
| Journal Title | Vajira Medical Journal |
| Journal Vol. | 62 |
| Journal No. | 3 |
| Keyword | Bile, Opisthorchis viverrini, Obstructive jaundice, Real-time PCR, Conventional PCR |
| URL Website | https://tci-thaijo.org/index.php/VMED |
| Website title | Vajira Medical Journal (วชิรเวชสาร) |
| ISSN | 0125-1252 |
| Abstract | Objective: To detect Opisthorchis viverrini (O. viverrini) DNA in bile samples from obstructive jaundice patients by real-time PCR assay and to compare the results of real-time PCR with conventional PCR. Methods: Bile samples were collected from 43 patients with obstructive jaundice from the Department of Surgery, Rajavithi Hospital and the Department of Surgery and Department of Diagnostic Radiology, Udon Thani Cancer Hospital. Twenty-eight cholangiocarcinoma (CCA) and 15 non-CCA patients underwent endoscopic retrograde cholangiography (ERCP) and biliary tract surgery. All bile samples were detected for O. viverrini DNA by real-time PCR and conventional PCR. The results of positive and negative bile samples for O. viverrini DNA detection by real-time PCR and conventional PCR detection were compared. Results: Thirteen (30.2%) out of 43 bile samples were O. viverrini DNA positive. Nine of 13 bile samples were positive by real-time PCR and conventional PCR, while 4 bile samples were positive only by real-time PCR. Thirty (69.8%) bile samples were negative for O. viverrini DNA by both methods. The sensitivity, specificity, positive and negative predictive values of the real-time PCR were 100%, 88.2%, 69.2% and 100%, respectively. Conclusion: Real-time PCR assay is suitable for detection of O. viverrini DNA in bile samples especially in obstructive jaundice patients. |
