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Confirmatory Semen Detection Using Real-Time PCRTechnique |
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| รหัสดีโอไอ | |
| Creator | Somruetai Satmun,Chachawin Rangabphai,Chairat Manasatienkij |
| Title | Confirmatory Semen Detection Using Real-Time PCRTechnique |
| Publisher | Text and Journal Publication |
| Publication Year | 2559 |
| Journal Title | Vajira Med |
| Journal Vol. | 60 |
| Journal No. | 1 |
| Page no. | 43-52 |
| Keyword | Real-time PCR, Messenger RNA, Protamine 2, Spermatozoa, DNA analysis |
| ISSN | 0125-1252 |
| Abstract | Objective: To study the specificity of real-time PCR technique for confirmatory semen detectionMethods: Eighteen samples each of blood, saliva, and semen were collected from 18 malevolunteers. All 54 samples were subjected to forensic DNA typing and mRNA analysis. DNAtemplates were amplified using the GlobalFiler? PCR amplification kit. For mRNA analysisprotamine 2 (PRM2), a sperm specific gene, was analyzed using ?-2-microglobulin gene (B2M), a cellular housekeeping gene, as the internal control.Results: DNA profiles of all blood, saliva and semen samples from the same volunteersdemonstrated exactly similar profiles. For mRNA analysis, B2M was expressed in all 54samples but only the 18 semen samples showed specific amplification with PRM2.Conclusions: PRM2 gene was a very effective marker for semen identification. Real-time PCRtechnique could be suitable for confirmatory detection of semen in medico-legal traceevidence collection. |
