|
Identification and genotyping of Chlamydia abortuswith MLVA from ruminant abortions in the Marmara region of Turkey |
|---|---|
| รหัสดีโอไอ | |
| Creator | Mehmet Engin MALAL |
| Title | Identification and genotyping of Chlamydia abortuswith MLVA from ruminant abortions in the Marmara region of Turkey |
| Contributor | Suheyla TURKYILMAZ |
| Publisher | Chulalongkorn University |
| Publication Year | 2564 |
| Journal Title | The Thai Journal of Veterinary Medicine |
| Journal Vol. | 51 |
| Journal No. | 1 |
| Page no. | 169 |
| Keyword | Chlamydia abortus, Genotyping, MLVA, Real Time PCR, VNTR, Turkey |
| URL Website | https://he01.tci-thaijo.org/index.php/tjvm/index |
| Website title | https://he01.tci-thaijo.org/index.php/tjvm/index |
| ISSN | 0125-6491 |
| Abstract | Abortion in ruminants isan important problem in Turkey and it leads to serious economic losses for farmers. The aim of this study is to diagnose Chlamydia abortus in ruminant abortions in the Marmara Region of Turkey and to determine the genotypes of the agent and obtain the first national epidemiological data in this sense. For this purpose, a total of 730 abortion materials (fetal tissue, fetal stomach contents, placentas, cotyledons, vaginal swabs)belonging to267 cattle, 380 sheep, 70 goats and 13 water buffaloes were examined for C. abortuswith species specific real-timePCR. DNAs of positive samples were genotyped by the Multilocus Variable Number Tandem Repeat Analysis (MLVA)method. From 730 materials, 87 (11.9%) were found positive for C. abortus. Positivity rates were 21.4% in goats, 16.6% in sheep, 7.7% in water buffaloes and 3% in cattle. The dominant genotype was found to beMLVA genotype 2 (93.1%), and 4 different genotypes includinggenotypes 3, 4, and 5 were involved in infections. So it is concluded that genotypic diversity of C. abortusis high in the Marmara region. It was also revealedthat C. abortuswas responsible for a significant proportionof small ruminant abortions inthis region. |
