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Study of the mechanism of Fomes officinalispolysaccharides inhibiting the contraction of the isolated duodenum in mice through the myenteric plexus |
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| รหัสดีโอไอ | |
| Creator | Ailong Sha |
| Title | Study of the mechanism of Fomes officinalispolysaccharides inhibiting the contraction of the isolated duodenum in mice through the myenteric plexus |
| Contributor | Haiyan Hao |
| Publisher | Chulalongkorn University |
| Publication Year | 2564 |
| Journal Title | The Thai Journal of Veterinary Medicine |
| Journal Vol. | 51 |
| Journal No. | 1 |
| Page no. | 7 |
| Keyword | Fomes officinalispolysaccharides, duodenal smooth muscle, G protein-coupled receptor, mechanism, myenteric plexus, Ca2+, neurotransmitters, signal pathways |
| URL Website | https://he01.tci-thaijo.org/index.php/tjvm/index |
| Website title | https://he01.tci-thaijo.org/index.php/tjvm/index |
| ISSN | 0125-6491 |
| Abstract | The objectives of this study were to observe the effect of different concentrations of Fomes officinalispolysaccharides (FOPs) on the contractile activity of the isolated duodenal smooth muscle in mice and explore its mechanism. The contractility of the isolated duodenum was recorded by the Medlab biological signal acquisition and processing system before and after administration and the effects of FOPs-treated groups at low, medium and high dose(5, 10 and 20 mg/mL) on the contractile frequency and amplitude of the intestines were observed. Adrenaline hydrochloride (AD) and CaCl2were selected respectively to be co-incubated with the high doseFOPsto observe the effects on duodenal contraction. The effects of different concentrations of the FOPson the activities of acetylcholine transferase (ChAT), acetylcholine Esterase (A-CHE), total nitric oxide synthase (TNOS)and the contentof Leucine-enkephalin (Leu-enk)in the isolated duodenum of the myenteric plexus in mice were detected by UV-Vis and enzyme-linked immunosorbent assay (ELISA). The results showed that the three FOPs-treatedgroupshad different inhibitory effects on the contractile frequency and amplitude of the isolated duodenum in mice compared with those before administration. The high doseFOPscould synergize (P<0.01)the inhibition effect of ADon the frequency and amplitude of the intestinal contraction and could significantly (P<0.01)inhibit the promotion effect of CaCl2on it. The effect was equivalent to that of isoprenaline hydrochloride (ISO) or verapamil hydrochloride, respectively. The results of UV-Vis and ELISA showed that compared with the control group, the activity of ChATand the content of Leu-enkin the three FOPs-treatedgroupsdecreased to varying degrees, contrarily, the activities of A-CHEand TNOS significantly increased (P<0.01). All the results suggest that the FOPs caninhibit the contraction of the isolated duodenum in mice, and themechanism of action is that theFOPs cannot onlyinhibit the signal transduction pathways of G protein-coupled M receptor-mediated AC-cAMP-PKA and PLC-IP3-Ca2+, but also the Ca2+signalling systems (such as inhibiting ICa-Lof the muscle membrane and then inhibiting Ca2+-CaM signalling pathway) through the myenteric plexus. By inhibiting the release of Leu-enk from the motor neurons of the myenteric plexus, the G protein-coupled delta receptor-mediated GTP-cAMP-(PKK or PKC) signalling pathway and Ca2+signalling systems were inhibited. By promoting the release of NOS from the motor neurons of the myenteric plexus, the increased NO was induced, then the enzyme-linked receptor-mediated GC-cGMP-PKG signal transduction pathway was reactivated. The G protein-coupled receptor-mediated AC-cAMP-PKA signal transduction pathway was activated by the myenteric plexus. |
