Genetic Relationship Assessment of Apples Using HAT-RAPD Technique
รหัสดีโอไอ
Creator NarumolThanananta
Title Genetic Relationship Assessment of Apples Using HAT-RAPD Technique
Contributor Kanittha Kittisubjareng, Theerachai Thanananta
Publisher Thammasat University
Publication Year 2567
Journal Title Thai Journal of Science and Technology
Journal Vol. 12
Journal No. 1
Page no. 1-8
Keyword apple, genetic relationship, high annealing temperature-random amplified polymorphic DNA (HAT-RAPD)
URL Website https://www.tci-thaijo.org/
Website title THAIJO
ISSN 2286-7333
Abstract High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) technique was used to genetic relationship assessment of 14 apple samples that sold in Thailand. Thirty-one random primers out of seventy-two were successful for DNA amplification. Then 20 random primers which gave clear amplified products were selected for DNA fingerprinting. The result showed significant differences among 14 samples and also showed some bands in each sample. Moreover, this research found 3 random primers which tended to be DNA markers for apple identification. A dendrogram constructed based on polymorphic bands showed genetic similarities among apples and separated to 3 clusters with similarity coefficients ranging from 0.57 to 0.86.
Thai Journal of Science and Technology

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