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Cherry Tomato Backcross Breeding to Increase Sweetness by dCAPS and SNPs Markers in Assisted Selection |
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| รหัสดีโอไอ | |
| Creator | Tonsak Rojkhamlue |
| Title | Cherry Tomato Backcross Breeding to Increase Sweetness by dCAPS and SNPs Markers in Assisted Selection |
| Contributor | Ornubol Chomdej, Pumipat Thongyoo, Julapark Chunwongse |
| Publisher | Thammasat University |
| Publication Year | 2566 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 11 |
| Journal No. | 4 |
| Page no. | 88-101 |
| Keyword | Tomato, Backcross breedingwith marker-assisted selection, SNP markers, Sweetness |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Cherry154 is typically consumed fresh, so the taste is essentially required. To enhance their sweetness, the Cherry154 containeda sweetness value at 6-8?Brix was subjected to backcrossing with Wva700 which contained 9-11?Brix. Normally, Backcross breeding is used to transfer 1-2 traits of interest from a donor parent to a recurrent parent. This process takes 5-6 generations to fixgenetic background from one to another variety. This research used the Kompetitive Allele Specific PCR technique by 200 SNPs marker-assisted backcrossing in each generation to aid in genetic background selection, covering every 5 cM on the tomato genome and analyzing data with the Flapjack program. It also used a dCAPs marker to select the LIN5gene in order to increase sweetness from backcrossing between Cherry154 and Wva700. The results showed plants in BC1F1had various recurrent parent percentage (RPP) values from 64-91%, BC2F1had RPP values varied from 91-81% and BC3F1were 91-99.6%. Plant in each generation were selected based on their sweetness and high value on RPP percentage. Therefore, the plant contained 8.3 ?Brix with 88.1% at BC1F1, 9.6?Brix with 89.8% at BC2F1, and 7.6 ?Brix with 95.6% RPP at BC3F1were chosen. It is indicated that a background selection using marker-assisted backcrossing allows plant breeding programs to be potentially precise for trait selection, time-saving, and meeting consumer needs. |
