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Efficiency for Shoot Multiplication of Srivijaya New Hybrid Pineapple Variety Using Three Tissue Culture Systems |
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| รหัสดีโอไอ | |
| Creator | Suneerat Sripaoraya |
| Title | Efficiency for Shoot Multiplication of Srivijaya New Hybrid Pineapple Variety Using Three Tissue Culture Systems |
| Contributor | Lenti Valentina,Pacharaporn Vanichpakorn, Pornsil Seephueak, Kamonwan Boonchareon, TanesChotikamas |
| Publisher | Thammasat University |
| Publication Year | 2566 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 11 |
| Journal No. | 4 |
| Page no. | 63-76 |
| Keyword | Srivijaya, pineapple, Ananas comosus, bioreactor, BA, liquid shake culture, solid culture |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | A new hybrid pineapple variety, 'Srivijaya, has superior fruit quality and is resistant to disease. The planting materials of the 'Srivijaya' pineapple variety are still limited for farmers. The tissue culture technique provides for rapid mass shoot production. This research, 'Srivijaya' pineapple tissue culturewas invested. Suckers were sterilizedusing70% ethanolfor 5 minutes and were moved to new bottle containing 40 ml of NaOCl (6 % w/w), 60 ml of distill water and 3 drops of tween-20for 35 minutes. The sucker was cut and culturedto induce shoots. Shoot multiplication of 'Srivijaya' pineapple was studied on three different in vitroculture systems; solid, liquid, and bioreactor. A completely randomized design was used with four treatments; 1/2 MS+ 0.5 mg/L BA, 1/2 MS+1 mg/L BA, 1/2 MS+ 2 mg/L BA and MS+ 2mg/L BA. The data were analyzed by ANOVA and DMRT for mean different analyses. The results showed that MS+ 2 mg/L BA medium gave the highest total number of shootsin all culture systems. For solid culture, 17.75, 21.50, 25 and 30.25 shoots were produced from 1/2 MS + 0.5, 1, 2 and MS+ 2 mg/L BA media respectively. Similar to liquid culture, 21.75, 23.50, 22.50 and 34.75 shoots were produced from four media respectively. The bioreactor was the most efficient in shoot multiplication of 'Srivijaya' for all treatments giving 51.5, 76, 95.50 and 124.5 shoots from 1/2 MS + 0.5, 1, 2 and MS+ 2 mg/L BA media respectively. |
