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Larvae stage of Thai freshwater pearl mussel, Hyriopsis (Hyriopsis)bialatus Simpson 1900,morphological development |
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รหัสดีโอไอ | |
Creator | Mayuva Youngsabanant |
Title | Larvae stage of Thai freshwater pearl mussel, Hyriopsis (Hyriopsis)bialatus Simpson 1900,morphological development |
Contributor | Uthaiwan Kovitvadhi, Satit Kovitvadhi |
Publisher | Thammasat University |
Publication Year | 2564 |
Journal Title | Thai Journal of Science and Technology |
Journal Vol. | 10 |
Journal No. | 6 |
Page no. | 635-648 |
Keyword | Freshwater pearl mussel, Glochidia, Hyriopsis bialatus, Juvenile, morphology |
URL Website | https://www.tci-thaijo.org/ |
Website title | THAIJO |
ISSN | 2286-7333 |
Abstract | Glochidia of Thai freshwater pearl mussel, Hyriopsis (Hyriopsis)bialatuswas cultured in an artificial medium consisting of M199, fish plasma (Cyprinus carprio), and antibiotics/antimycotic at a ratio of 2:1:0.5 (V/V). They were reared at a density of 50-100 glochidia per dish under sterile conditions until they transformed into juveniles. The culture dish was incubated at 23?2?C with a constant supply of 5% CO2. Theduration of glochidia development until the juvenile stage was 10 days. After transformation, early juvenile mussels were reared and fed with a mixture of 4 types of phytoplankton (Chlorella sp.2., Kirchneriella incuvata, Naviculasp.and unknown) until 15 days. The morphological study of glochidia and early juveniles was carried out using a light and scanning electron microscope. The results illustrated that glochidia and early juveniles cultured in an artificial medium exhibited normal morphological development and could undergo normal transformation. The appearance of primitive gills, foot, gastrointestinal tract, and growth lines on the shell are signs of complete metamorphosis. |