Larvae stage of Thai freshwater pearl mussel, Hyriopsis (Hyriopsis)bialatus Simpson 1900,morphological development
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Creator Mayuva Youngsabanant
Title Larvae stage of Thai freshwater pearl mussel, Hyriopsis (Hyriopsis)bialatus Simpson 1900,morphological development
Contributor Uthaiwan Kovitvadhi, Satit Kovitvadhi
Publisher Thammasat University
Publication Year 2564
Journal Title Thai Journal of Science and Technology
Journal Vol. 10
Journal No. 6
Page no. 635-648
Keyword Freshwater pearl mussel, Glochidia, Hyriopsis bialatus, Juvenile, morphology
URL Website https://www.tci-thaijo.org/
Website title THAIJO
ISSN 2286-7333
Abstract Glochidia of Thai freshwater pearl mussel, Hyriopsis (Hyriopsis)bialatuswas cultured in an artificial medium consisting of M199, fish plasma (Cyprinus carprio), and antibiotics/antimycotic at a ratio of 2:1:0.5 (V/V). They were reared at a density of 50-100 glochidia per dish under sterile conditions until they transformed into juveniles. The culture dish was incubated at 23?2?C with a constant supply of 5% CO2. Theduration of glochidia development until the juvenile stage was 10 days. After transformation, early juvenile mussels were reared and fed with a mixture of 4 types of phytoplankton (Chlorella sp.2., Kirchneriella incuvata, Naviculasp.and unknown) until 15 days. The morphological study of glochidia and early juveniles was carried out using a light and scanning electron microscope. The results illustrated that glochidia and early juveniles cultured in an artificial medium exhibited normal morphological development and could undergo normal transformation. The appearance of primitive gills, foot, gastrointestinal tract, and growth lines on the shell are signs of complete metamorphosis.
Thai Journal of Science and Technology

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