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Antioxidant Activities and Cytotoxicity Screening of the Extracts from Indigo (Indigofera tinctoria) |
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| รหัสดีโอไอ | |
| Creator | Yada Poonkasem |
| Title | Antioxidant Activities and Cytotoxicity Screening of the Extracts from Indigo (Indigofera tinctoria) |
| Contributor | Somchit Damrianant and Niramol Sakkayawong |
| Publisher | Thammasat University |
| Publication Year | 2564 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 10 |
| Journal No. | 5 |
| Page no. | 571-586 |
| Keyword | Indigo, Indigofera tinctoria, antioxidant activity, cytotoxicity screening |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Antioxidant activities and cytotoxicityscreeningof the ethanol extracts from different parts of Indigo(Indigofera tinctoria) including mature leaves, immature leaves and seeds, were investigated. All samples were preparedby Soxhlet extractions with absolute ethanol. Total phenolic content (TPC) by Folin-Ciocalteu method and radical scavenging analyses by DPPH (1,1diphenyl-2-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) were employed. The levels of TPC werehigh in the crude extracts from mature and immature leaves, 12.34ฑ2.59and 12.90ฑ3.13mg GAE/g dried weight, respectively, while antioxidant activity by FRAP was increased in that fromthe seeds, 135.21ฑ15.16?mol/g dried weight. The IC50(half maximal inhibitory concentration) valuesby DPPH werelow in the seed extracts, 0.23ฑ0.10mg/mL. The major compounds in the extracts were further identified by thin layer chromatography (TLC). The leaf extracts were displayedto contain rutin, quercetin, kaempferol, and apigenin. In addition, epicatachin and gallic acid were unveiled in the seed extracts. Cytotoxic assaysto human dermal fibroblasts-adult (HDFa) shown by ED50(half maximal effective dose) values,in the extracts from mature leaves, immature leaves and seeds were 1.14x 103, 1.55x 103and 4.26x 103?g/mL, respectively. It is suggested that the ethanol extracts from Indigo possessavaluable aspectto bedeveloped as a potential phytomedicine. |
