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Morphological and Molecular Identification of the Predatory Nematode, Mononchoides (Nematoda:Diplogasterida) from Thailand |
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| รหัสดีโอไอ | |
| Creator | Arunee Suwanngam |
| Title | Morphological and Molecular Identification of the Predatory Nematode, Mononchoides (Nematoda:Diplogasterida) from Thailand |
| Contributor | Anongnuch Sasnarukkit, Raktapa Phonginsee, Buncha Chinnasri, Srimek Chowpongpang |
| Publisher | Thammasat University |
| Publication Year | 2564 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 10 |
| Journal No. | 2 |
| Page no. | 173-184 |
| Keyword | Mononchoides, predatory nematodes, specific primer |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Predatory nematodes; Mononchoides are the beneficial nematodes that feed on small organisms or other nematodes. Mononchoides population collected from guava orchards and para rubber plantation in Thailand was characterized by morphological and molecular techniques. Mononchoides specie is characterized by a dorsal claw-like tooth; 7-8 ?m wide and 3-4 ?m long, long body (540-650 ?m), didelphic-amphidelphic reproductive system and a relatively short filiform tail (40-50?m long). Genetic analysis of Mononchoides based on species-specific primers and sequence on the ribosomal DNA. This molecular diagnosis using Mnc1, Mnc3 and Mn28s primers were confirmed by nucleotide sequences that shared 100% similarity with the known nematode sequences (FJ661080, LC210629, KT884893, LN827618, KJ877211, KF151166, KP067833) in GenBank database. The Mononchoides-specific primers have been developed to offered a rapid species identification approach for further analysis and management. |
