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Genetic Relationship Assessment of Indigofera Using Nucleotide Sequences of matK Gene |
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| รหัสดีโอไอ | |
| Creator | Supharat Buaban |
| Title | Genetic Relationship Assessment of Indigofera Using Nucleotide Sequences of matK Gene |
| Contributor | Niramol Sakkayawong, Theerachai Thanananta, Narumol Thanananta |
| Publisher | Thammasat University |
| Publication Year | 2563 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 9 |
| Journal No. | 4 |
| Page no. | 577-586 |
| Keyword | Indigofera, genetic relationship, nucleotide sequence, matK |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | The objective of this research was to sequence the nucleotides of matK gene in order to identify 15 samples of Indigofera plant. This is a fast, timesaving, and low-cost method. When comparing nucleotide sequences with data in NCBI database, 3 species of Indigofera, which are Indigofera tinctoria, Indigofera tinctoria var. arrecta, Indigofera caroliniana and Indigofera suffruticosa, were identified. But it was not able to specify species for the sample no. 13. The data were analyzed with ClustalW and made a phylogenetic tree using UPGMA by MEGA version X program. It was found that the Indigofera samples were divided into 2 groups with genetic differences in a range of 0.105-0.389. Therefore, nucleotide sequences of other genes should be used for more efficiency. The information obtained from this research can be applied in the conservation and breeding of Indigofera plant. |
