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การพัฒนากระบวนการเตรียมสารสกัดสมุนไพรไทยที่มีฤทธิ์ต้านอนุมูลอิสระ |
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| รหัสดีโอไอ | |
| Creator | ศรัณยู อุ่นทวี |
| Title | การพัฒนากระบวนการเตรียมสารสกัดสมุนไพรไทยที่มีฤทธิ์ต้านอนุมูลอิสระ |
| Contributor | ชนัญ ผลประไพ |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 5 |
| Page no. | 479-492 |
| Keyword | Sappanwood, Indian gooseberry, anti-oxidant activity, non-communicable disease |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | In the last decade, non-communicable diseases (NCDs) are the major causes of premature mortality in Thailand. Mitochondrial alterations, oxidative stress and inflammation have been reported to be the important roles in onset and development of NCDs. These abnormalities in the cells are mainly caused by free radical or reactive oxygen species (ROS). Thus, this research aimed to develop the extraction process for preparing high anti-oxidant extracts from Thai herbs. To screen the potent herbal plants, Sappanwood heartwood (SH), Indian gooseberry fruit (IG), Chebulic myrobalan fruit (CM), Belliric myrobalan fruit (BM) and heart-leaved moonseed stem (HM) were extracted by macerating with ethanol. The selected herbs were macerated in various solvents to optimize the most suitable solvent for the extraction. The results revealed that SH extract and IG extract exhibited high potential for anti-oxidant activity among other extracts due to these extracts were determined to have low inhibitory concentration at 50% (IC50) as respective 5.13 ? 0.75 and 5.18 ? 0.69 ?g/mL. Additionally, total phenolic content (TPC) of SH extract and IG extract were found to be high as 699.68 ? 34.02 and 369.42 ? 5.39 mg gallic acid equivalent (GAE)/ g extract, respectively. After the selected herbs were macerated in various solvents including methanol (MeOH), ethanol (EtOH) and ethyl acetate (EtOAc), the results had showed that the most suitable solvent was EtOH. The IC50 values and TPC values of SH-EtOH and IG-EtOH were calculated to be 3.40 ? 0.42 and 2.35 ? 0.22 ?g/mL, 854.12 ? 41.01 and 598.33 ? 14.66 mg GAE/ g extract. For total flavonoid content (TFC) of SH-EtOH and IG-EtOH, it were quantified to be 170.21 ? 14.20 and 276.08 ? 6.79 mM Quercetin equivalent/ g extract, and 843.87 ? 46.35 and 102.75 ? 18.37 mM Catechin equivalent/ g extract, respectively. Although extraction of SH and IG by using EtOH gave the extraction yield lower than that MeOH, it is low toxic and low cost. These make EtOH more suitable for extracting SH and IG in community and industrial scales. The extraction process we developed could increase the value of herbal plants and enabled the potency of herbal extracts in various pharmacological utilizations, especially for intensive study on NCDs. |
