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การวิเคราะห์ลำดับนิวคลีโอไทด์ของนิวเคลียร์ไรโบโซมอลไอทีเอสในบัวประดับสกุล Nymphaea ของประเทศไทย |
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| รหัสดีโอไอ | |
| Creator | ณัฐชา แสงศรี |
| Title | การวิเคราะห์ลำดับนิวคลีโอไทด์ของนิวเคลียร์ไรโบโซมอลไอทีเอสในบัวประดับสกุล Nymphaea ของประเทศไทย |
| Contributor | จตุพร กุลอึ้ง, วิภา หงษ์ตระกูล |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 3 |
| Page no. | 258-270 |
| Keyword | waterlily, nrITS, phylogenetic tree |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Sequences of nrITS were analyzed in order to evaluate genetic variation in 14 Thailand waterlilies, Nymphaea spp. using BioEdit program. The ITS (Internal Transcribed Spacer) region of approximately 900 bp contains sequences of partial 18S gene, ITS1, 5.8S gene, ITS2 and partial 26S gene. Nucleotide sequence variations at ITS1 and ITS2 were observed more than at 18S, 5.8S and 26S genes. Nucleotide lengths of ITS1 were longer than ITS2, but GC contents of ITS1 were less than ITS2. The 5.8S gene sequence was 156 bp in all 14 waterlily samples. Maximum likelihood (ML) phylogenetic tree of 14 waterlilies was constructed based on nrITS sequences using MEGA6 program. The tree could classified 14 waterlilies into 5 groups of different subgenus, that are subgenus Anecphya, subgenus Brachyceras, Intersubgeneric hybrids (ISG) of subgenus Nymphaea and subgenus Brachyceras, subgenus Lotos and subgenus Nymphaea. The classification was consistent with botanical classification in waterlily. |
