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การชักนำให้เกิดแคลลัสจากชิ้นส่วนต่าง ๆ ของต้นลินเดอร์เนียในสภาพปลอดเชื้อ |
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| รหัสดีโอไอ | |
| Creator | กาพย์แก้ว แก้วนาบอน |
| Title | การชักนำให้เกิดแคลลัสจากชิ้นส่วนต่าง ๆ ของต้นลินเดอร์เนียในสภาพปลอดเชื้อ |
| Contributor | ธัญญะ เตชะศีลพิทักษ์, ทัศไนย จารุวัฒนพันธ์, ณัฐพงค์ จันจุฬา, นุชรัฐ บาลลา |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 2 |
| Page no. | 138-145 |
| Keyword | leaf, cotyledon, internode, tissue culture |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Lindernia is a flowering plant with high genetic diversity. This plant can survive in almost any environment and flowers all year round, making it to be suitable for improving as an ornamental plant for the future. In plant regeneration, BA and NAA are useful for shoot and internode inductions. The aim of this research was to study the regeneration potential of Lindernia explants (cotyledon, leaf, and internode) on ? MS media with BA at concentrations ranging from 0.5-2.0 mg/L) in in vitro culture. Callus sizes and shoots were recorded for 4 weeks. The results showed that the explants cultured in BA 2 mg/L had the highest shoot induction rate (17.91, 19.1 and 18.67 shoots/explant, respectively). Three parts of the plant were cultured in BA medium at 1.0 mg/L with NAA at concentrations in a range of 0.5-2.0 mg/L. It was found that cotyledons were cultured on 1.0 mg/L BA and 1.5 mg/L NAA. Leaf pieces were cultured on 1.0 mg/L BA with NAA 0.5 mg/L. And the segments were cultured on 1.0 mg/L BA with NAA 0.5 mg/L. The average numbers of shoots were 18.08, 16.91 and 10.50 per shoot, respectively. |
