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Molecular Cloning of Arsenate reductase and Phytochelatin synthase gene related to arsenic response in Oryza sativa L. var. indica cv. Khao Dawk Mali 105 |
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| รหัสดีโอไอ | |
| Creator | 1. Pawonrat Patipipat 2. Pattana Srifah Huehne 3. Kisana Bhinija 4. Bhawat Wongkhamprai |
| Title | Molecular Cloning of Arsenate reductase and Phytochelatin synthase gene related to arsenic response in Oryza sativa L. var. indica cv. Khao Dawk Mali 105 |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2557 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 7 |
| Journal No. | 1 |
| Page no. | 41 |
| Keyword | Oryza sativa, Khao Dawk Mali 105, arsenic, arsenate reductase, phytochelatin synthase |
| ISSN | 8578664 |
| Abstract | Accumulation of arsenic (As) in plants can severely inhibit plant growth. Once the arsenic is taken up by roots, arsenic detoxification enzymes such as arsenate reductase (AR) and phytochelatin synthase (PCS) are up-regulated in response to arsenic accumulation. In this study, the two-detoxification enzymes were cloned from roots of arsenic-treated Oryza sativa L. ssp. indica, cv. Khao Dawk Mali 105 (Jasmine rice). The results showed that the AR gene consists of 414 bp encoding a polypeptide with 137 amino acids long. This encoded amino acid sequence contains the consensus motif HisCys(X)5Arg that is essential for reductase activity and is similar to the arsenate reductase polypeptide from African rice (Oryza glaberrima Steud.). Moreover, the partial phytochelatin synthase gene at 3' end, 1,498 bp containing 873 coding sequence, was successfully cloned. The obtained phytochelatin synthase gene encoding for a 290 amino acid polypeptide was highly related to phytochelatin synthase from Triticum aestivum L. The deduced amino acid sequence contained consensus motif CysCys(X)3Cys(X)2Cys at C-terminal which serves as a sensor for heavy metal ions and arsenic. |
