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การตรวจการกลายของยีน BRAF ในโรคมะเร็งต่อมไทรอยด์ (Molecular Testing of BRAF Mutation in Thyroid Cancer) |
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| รหัสดีโอไอ | |
| Creator | อัญชลี ไหลพิริยกุล |
| Title | การตรวจการกลายของยีน BRAF ในโรคมะเร็งต่อมไทรอยด์ (Molecular Testing of BRAF Mutation in Thyroid Cancer) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 147 |
| Keyword | BRAF mutation, thyroid cancer, DHPLC |
| ISSN | 8578664 |
| Abstract | Thyroid carcinoma is the most prevalent form of malignant endocrine tumor. The most common type of which is papillary thyroid carcinoma (PTC). Several studies suggested the correlation and specificity of papillary thyroid carcinoma with BRAF V600E mutation. The laboratory detection of BRAF V600E mutation in PTC samples can assist clinicians for treatment planning, prognostication and diagnosis of thyroid cancer. The objective of this study was to develope DHPLC technique to detect BRAF V600E mutation in PTC. We performed the assay in 50 FFPE samples and 18 FNA samples from thyroid patients. Then, the extracted DNA was detected for BRAF V600E mutation by DHPLC and confirmed by direct sequencing. The result showed that DHPLC gave the same results of BRAF V600E. DHPLC is an alternative promising tool for detecting BRAF V600E mutation. |
