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การใช้ดีเอ็ชพีเอลซีสาหรับตรวจคัดกรองการกลายของยีน CEBPA ในผู้ป่วยโรคมะเร็งเม็ดโลหิตขาวชนิดมัยอิลอยด์แบบเฉียบพลัน (The Use of DHPLC for Mutation Screening of CEBPA Gene in Acute Myeloid Leukemia) |
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| รหัสดีโอไอ | |
| Creator | สุธาดา มากเมือง |
| Title | การใช้ดีเอ็ชพีเอลซีสาหรับตรวจคัดกรองการกลายของยีน CEBPA ในผู้ป่วยโรคมะเร็งเม็ดโลหิตขาวชนิดมัยอิลอยด์แบบเฉียบพลัน (The Use of DHPLC for Mutation Screening of CEBPA Gene in Acute Myeloid Leukemia) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 152 |
| Keyword | CEBPA, mutation, acute myeloid leukemia, DHPLC, screening |
| ISSN | 8578664 |
| Abstract | The mutations of CCAAT/enhancer binding protein-alpha (CEBPA) gene are valuated as a favorable prognostic tool for AML patients. The gold standard method for detection of CEBPA gene mutations is direct sequencing. However, this method has some disadvantages and CEBPAmutations can occur across the whole gene. Thus, it should have a screening test before designating the type of mutation by direct sequencing. This screening method requires an accurate, fast and easy method. The purpose of this study was to evaluate the ability of denaturing high-performance liquid chromatography (DHPLC) method for screening CEBPA mutations. The coding region of CEBPA gene in 40 AML patients was screened by DHPLC and confirmed by direct sequencing. Our results suggested that DHPLC was a useful screening test to detect CEBPAgene mutations in AML patients. |
