|
ความหลากหลายทางพันธุกรรมของพืชสกุล Passiflora (Genetic Diversity in Passiflora spp.) |
|---|---|
| รหัสดีโอไอ | |
| Creator | อนุรักษ์ โพธิ์เอี่ยม |
| Title | ความหลากหลายทางพันธุกรรมของพืชสกุล Passiflora (Genetic Diversity in Passiflora spp.) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 214 |
| Keyword | Passiflora spp., genetic diversity |
| ISSN | 8578664 |
| Abstract | Passiflora spp. is an economically important genus that shows great phenotypic variation. The objective of this work was to determine the diversity of Passiflora spp. based on the morphology of the leaves and molecular techniques. Seventeen of Passiflora spp. were collected from different countries in the Plant Genetic Conservation Project under the Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn, at the Suan pathum palace, Pathumthani Province. Including, Ka-thok-rok (P. foetida) and Saowarot (passion fruit: P. edulis var. Flavicarpa) found in Thailand. The Passiflora spp. shows a wide range of variation in their leaf morphology which was divided into 4 groups. For molecular technique, genetic diversity were investigated by PCR and nucleotide sequencing technique based on the internal transcribed spacers (ITS) regions of ribosomal DNA (rDNA) using the ITS1/ITS2 Passiflora specific primers. The PCR amplification of this region was detected a unique fragment of approximately 650 bp. Nineteen sequences were aligned and compared with public sequences available in the GenBank database. The nucleotide sequences were analyzed against those of the sequences using the PHYLIP software for phylogenetic analysis. The neighbour-joining dendrogram was generated which provided more information on polymorphism. Passiflora spp. was revealed a very high genetic diversity. In the construction of phylogenetic trees from DNA sequence data show four distinct divisions corresponded with leaf shape. It was found that P. edulis and P. foetida are placed in the same group. |
